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. 2022 Jul 19;10:915702. doi: 10.3389/fbioe.2022.915702

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Copyright © 2022 Jones, Di Cio, Connelly and Gautrot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histology sections of microvascularized skin-on-chip constructs and human skin sections immuno-stained with phalloidin (red), DAPI (blue), and for K14 and involucrin, (A) green and cyan, respectively or vimentin and transglutaminase, (B) green and cyan, respectively. Tissues were cultured for 14 days in submerged conditions. Scale bars: 50 μm. (C) Quantification of the corresponding K14+ tissue thickness , involucrin + tissue thickness (D) and transglutaminase + tissue thickness (E). **p < 0.01; ***p < 0.001; n.s., non-significant, p > 0.05; error bars are standard errors; n = 3.