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. 2022 Jul 19;13:939940. doi: 10.3389/fimmu.2022.939940

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Copyright © 2022 Liang, Xu, Liu, Li, Wang, Li, Liao, Lu, Li, Mu, Sun and Zhu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD4⁺ TCR-T clusters showed temporal transcriptome profiles, mixed Th1/Th2 phenotypes and unique cytotoxic signatures. (A)UMAP projection of the expression profiles of the 7825 CD4⁺ TCR-T cells. (B) UMAP manifolds colored by sample origins. (C) Stacked bar chart of sample origins in each CD4⁺ TCR-T cluster. (D) GO pathway analysis of CTL1/2/3/4 compared with cluster U. Top 30 DEGs were used for analysis. Top 5 GO-BP terms were displayed. The color key from red to blue indicates P values (P < 0.05) from low to high. Count: The number of genes enriched to this GO entry from the input gene for enrichment analysis. (E) Pseudotime trajectory plot of CD4⁺ TCR-T cells across the pseudotime trajectory. (F) Violin plots show expression of selected transcription factors and costimulatory molecules in CD4⁺ TCR-T clusters. (G) Dot plots show expression of representative cytotoxic genes in CD4⁺ TCR-T clusters. (H) Kaplan-Meier overall survival curves of TCGA SKCM patients grouped by LTA expression. P value was calculated by a log-rank test. DEG, differential expressed gene; GO, gene ontology; UMAP, uniform approximation and projection; BP, biological process.