TABLE 3.
Summary of accuracy, precision, recovery, and matrix effect of the analytes in rat plasma (n = 6).
| Analyte | Concentration (ng/ml) | Precision and accuracy | Recovery (%) | Matrix effect (%) | |||||
|---|---|---|---|---|---|---|---|---|---|
| Intra-day (%) | Inter-day (%) | ||||||||
| RSD | RE | RSD | RE | Mean | RSD | Mean | RSD | ||
| BF | 5.00 | 9.8 | 6.4 | 3.3 | 6.1 | 63.1 | 6.9 | 94.8 | 3.7 |
| 50.00 | 6.5 | −0.4 | 3.4 | −1.4 | 58.4 | 4.3 | 88.7 | 1.8 | |
| 249.99 | 7.6 | 0.2 | 4.1 | −1.6 | 63.9 | 9.8 | 93.1 | 11.2 | |
| BL | 5.00 | 9.5 | −2.0 | 4.2 | 1.2 | 52.8 | 9.1 | 105.2 | 10.9 |
| 50.05 | 7.1 | 2.1 | 5.8 | −3.5 | 71.9 | 3.2 | 109.7 | 1.1 | |
| 250.23 | 5.1 | 7.2 | 2.2 | 6.8 | 90.9 | 9.2 | 110.6 | 10.0 | |
| HBG | 5.00 | 7.4 | 10.2 | 5.0 | 4.5 | 54.2 | 4.2 | 109.9 | 10.7 |
| 49.97 | 11.0 | −2.8 | 4.1 | 0.2 | 69.2 | 4.6 | 113.8 | 4.6 | |
| 249.85 | 10.0 | −2.4 | 4.1 | 0.8 | 83.7 | 7.7 | 110.9 | 7.7 | |
| CBT | 5.00 | 11.3 | 2.4 | 3.7 | 6.9 | 58.2 | 13.9 | 89.1 | 11.6 |
| 50.05 | 7.0 | −0.8 | 6.7 | 7.2 | 55.6 | 6.8 | 92.0 | 5.6 | |
| 250.25 | 8.8 | 4.2 | 3.0 | 4.5 | 65.4 | 4.4 | 96.5 | 11.8 | |
| CBG | 5.00 | 9.3 | 5.0 | 4.4 | 0.2 | 57.3 | 9.7 | 110.9 | 3.5 |
| 50.02 | 7.8 | 6.7 | 8.1 | −2.0 | 55.6 | 3.3 | 113.4 | 5.7 | |
| 250.10 | 9.4 | 0.2 | 2.7 | 2.2 | 71.3 | 8.2 | 97.3 | 3.9 | |
| DCT | 5.00 | 9.4 | 5.7 | 2.8 | 5.0 | 97.8 | 6.6 | 89.4 | 4.2 |
| 49.99 | 8.5 | 2.2 | 7.1 | −4.1 | 86.7 | 3.2 | 96.9 | 3.8 | |
| 249.95 | 12.3 | −12.5 | 9.6 | −5.2 | 92.3 | 2.4 | 107.0 | 1.8 | |
| DCG | 5.00 | 7.9 | 11.4 | 7.9 | 2.4 | 57.8 | 4.7 | 111.4 | 13.0 |
| 50.06 | 14.3 | −2.6 | 3.5 | −3.5 | 82.6 | 3.4 | 106.7 | 3.0 | |
| 250.28 | 9.0 | −5.5 | 6.1 | 1.5 | 96.6 | 9.5 | 108.9 | 10.3 | |
| GBL | 5.00 | 10.4 | 11.3 | 0.6 | 10.5 | 52.7 | 4.8 | 106.4 | 6.5 |
| 49.98 | 7.6 | −7.1 | 3.0 | −3.8 | 56.5 | 4.0 | 107.8 | 2.7 | |
| 249.92 | 8.9 | −8.6 | 4.7 | −3.3 | 72.9 | 0.7 | 105.1 | 4.6 | |
| ABG | 5.00 | 6.6 | 12.6 | 6.9 | 6.8 | 61.9 | 4.0 | 111.2 | 2.0 |
| 49.97 | 10.2 | −3.6 | 2.0 | −5.8 | 62.2 | 5.8 | 110.2 | 5.8 | |
| 249.84 | 7.1 | −5.3 | 2.5 | −2.6 | 80.3 | 3.4 | 109.0 | 3.7 | |
| TCG | 5.00 | 9.8 | 11.9 | 1.5 | 11.6 | 52.0 | 6.5 | 104.4 | 3.9 |
| 50.05 | 7.7 | −2.0 | 4.3 | −2.9 | 69.6 | 1.8 | 100.8 | 0.9 | |
| 250.25 | 3.9 | −4.3 | 3.9 | −2.4 | 87.2 | 9.7 | 103.0 | 10.4 | |
| PBG | 5.00 | 5.8 | 13.0 | 5.1 | 8.1 | 64.7 | 11.7 | 95.8 | 13.7 |
| 50.04 | 6.7 | −0.3 | 3.9 | 1.7 | 65.4 | 4.2 | 104.6 | 7.4 | |
| 250.20 | 10.0 | 4.7 | 6.6 | 2.6 | 80.7 | 1.0 | 92.3 | 5.5 | |
| RBG | 4.99 | 7.9 | 4.1 | 4.4 | 2.1 | 80.2 | 3.7 | 107.4 | 0.6 |
| 49.92 | 9.4 | 6.1 | 5.7 | 1.1 | 68.8 | 2.7 | 110.6 | 0.9 | |
| 249.58 | 8.2 | −1.8 | 0.8 | −1.1 | 89.0 | 6.0 | 96.5 | 4.6 | |
| BTI | 5.00 | 9.7 | 3.4 | 2.8 | 3.4 | 4.3 | 5.1 | 93.7 | 10.7 |
| 49.98 | 9.0 | −6.1 | 3.8 | −4.0 | 4.0 | 12.1 | 90.2 | 8.1 | |
| 249.90 | 11.7 | −3.2 | 3.8 | 1.2 | 6.2 | 4.7 | 90.8 | 10.3 | |