TABLE 4.

Stability of 13 analytes in the rat plasma at three QC levels (n = 6).

Analyte	Concentration (ng/ml)	Stability during 24 h at 4°C		Three freeze–thaw cycles		−80°C for 30 days
		RSD (%)	RE	RSD (%)	RE (%)	RSD (%)	RE (%)
BF	5.00	12.0	3.3	7.9	−7.7	5.7	8.7
	50.00	5.3	−8.6	7.0	−10.1	4.3	−12.6
	249.99	12.5	−8.5	12.0	0.8	7.5	1.4
BL	5.00	5.4	11.4	9.0	3.4	9.0	2.3
	50.05	6.3	5.0	7.7	3.1	7.7	2.1
	250.23	6.2	5.7	3.4	7.1	3.4	6.0
HBG	5.00	7.2	10.2	8.9	9.3	7.4	−5.0
	49.97	8.1	−4.7	12.0	−2.1	8.0	8.9
	249.85	12.5	0.8	8.4	1.5	13.4	−1.3
CBT	5.00	7.6	9.8	10.0	4.3	9.4	−0.3
	50.05	7.9	7.7	5.1	13.2	9.4	4.1
	250.25	12.9	4.8	10.5	12.0	7.6	−8.7
CBG	5.00	8.1	4.8	6.4	4.8	6.4	3.7
	50.02	7.8	−3.2	7.6	0.0	7.6	−1.0
	250.10	11.3	0.6	12.2	3.4	12.2	2.3
DCT	5.00	5.5	11.4	11.2	3.7	13.2	2.4
	49.99	11.3	7.3	6.5	12.1	5.6	12.7
	249.95	7.0	9.6	7.9	7.3	8.6	8.7
DCG	5.00	13.5	1.5	11.3	3.0	11.3	1.9
	50.06	6.4	6.7	8.0	4.9	8.0	3.8
	250.28	4.5	4.6	5.9	−2.8	5.9	−3.8
GBL	5.00	6.2	12.0	2.1	13.2	2.2	8.3
	49.98	10.6	10.9	5.4	10.7	6.1	12.4
	249.92	7.9	5.4	7.7	−0.6	8.0	−4.9
ABG	5.00	12.8	7.4	10.3	2.5	10.2	7.1
	49.97	12.5	−5.3	12.5	3.3	10.7	8.3
	249.84	11.2	−4.1	11.1	0.7	11.9	2.3
TCG	5.00	11.2	−0.3	10.6	5.8	8.7	0.9
	50.05	12.8	−4.2	5.7	0.0	7.6	3.8
	250.25	3.7	−4.7	8.2	-6.6	7.9	−2.1
PBG	5.00	11.0	6.6	11.1	4.4	6.9	8.3
	50.04	9.9	12.0	5.0	13.3	6.4	7.5
	250.20	9.7	10.2	11.5	7.7	11.6	4.7
RBG	4.99	7.1	−6.4	9.1	−0.3	9.1	−1.3
	49.92	8.9	3.5	11.7	3.3	11.7	2.3
	249.58	9.5	−3.3	7.5	−1.9	7.5	−2.8
BTI	5.00	8.1	−0.3	10.4	−3.4	9.7	1.7
	49.98	7.7	−9.3	5.3	2.5	9.0	−4.1
	249.90	5.0	−5.6	5.3	5.0	9.0	3.0