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. 1999 Feb;181(3):748–756. doi: 10.1128/jb.181.3.748-756.1999

FIG. 1.

FIG. 1

Physical and genetic map of Tn5-OT182 and various cepIR constructs. (A) Tn5-OT182 (38). The arrows represent the orientation and position of genes, and the black box represents the pBR325 origin of replication. Designations and abbreviations: lacZ, promoterless β-galactosidase reporter gene; bla, β-lactamase gene; ori, origin of replication; tetRA, gene encoding tetracycline resistance determinant; tnp, gene encoding transposase; B, BamHI; C, ClaI; Ss, SstI; E, EcoRI; X, XmnI; Sa, SalI; S, StuI; H, HindIII; Xh, XhoI. (B) The cepIR locus from B. cepacia (pSLA3.2). The arrows represent the location and orientation of genes, and the “lollipop” represents the site of transposon insertion. Designations and abbreviations: pSLR100, 1.65-kb cepR subclone in pUCP28T; pSLS201, 1.55-kb cepI subclone in pNOT19; pSLS201-T, trimethoprim cassette (tmp) introduced into pSLS201; cepI, gene encoding autoinducer synthase; cepR, gene encoding transcriptional activator; Sp, SphI; C, ClaI; A, AccI; K, KpnI; P, PstI; Xh, XhoI.