TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 56 |
| SM10 | Mobilizing strain; RP4 tra genes integrated in chromosome; Kmr | 62 |
| MG4 | Δ(argF-lac)U169 zah-735::Tn10 recA56 srl::Tn10 | 53 |
| HB101 | supE44 hsdS20(rB mB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 | 56 |
| VJS533 | recΔA56 ara Δ(lac-proAB)X111 rpsL φ80dΔ(lacZ)M15 | 70 |
| B. cepacia | ||
| K56-2 | Cystic fibrosis respiratory isolate | 9 |
| K56-R2 | cepR::Tn5-OT182 derivative of K56-2 | This study |
| K56-I2 | cepI::tmp derivative of K56-2 | This study |
| R. solanacearum AW1-A18 | solI8::SP | 19 |
| Plasmids | ||
| pOT182 | pSUP102(GM)::Tn5-OT182 Cmr Gmr Apr Tcr | 38 |
| pNOT19 | Modified pUC19 cloning vector; Apr | 57 |
| pUCP28T | Broad-host-range vector; IncP OriT pRO1600 ori Tpr | 58 |
| p34E-Tp | Source of tmp cassette; Tpr | 11 |
| pRK2013 | ColE1 Tra (RK2)+ Kmr | 18 |
| pEX18Tc | Suicide vector; sacB Tcr | 26 |
| pCRR2.1TOPO | Cloning vector for PCR products | Invitrogen |
| pSLR2-1 | 11.3-kb ClaI fragment from K56-R2 obtained by self-cloning; Tcr | This study |
| pSLR2-2 | 11.5-kb XhoI fragment from K56-R2 obtained by self-cloning; Tcr | This study |
| pSLA3.2 | pUCP28T with 3.2-kb SphI fragment from K56-2 containing cepIR genes; Tpr | This study |
| pSLR100 | pUCP28T with 1.65-kb KpnI-SphI fragment from pSLA3.2 containing cepR gene; Tpr | This study |
| pSLS201 | pNOT19 with 1.55-kb SphI-KpnI fragment from pSLA3.2 containing cepI gene; Apr | This study |
| pSLS201-T | pNOT19 with 2.2-kb fragment containing cepI gene inactivated with tmp cassette; Apr Tpr | This study |
| pEXCEPI | PCR product (inactivated cepI fragment from pSLS201-T) in pEX18Tc; Tcr Tpr | This study |
| pKDT17 | lasB::lacZ plac-lasR Apr | 48 |
| pHV2001− | luxR luxI′CDABE Apr | 49 |
| p395B | aidA::lacZ Tcr | 19 |
| pECP61.5 | rhlR rhlA::lacZ Apr | 51 |