Fig. 2.

Basic parameters as well as extensive behavioural testing did not indicate changes between the treatment groups. Survival curves showed no significant increase in mortality in any of the treatment groups (A). Animals typically lost weight during the first week after tMCAO and fully recovered until 28 dpi (B). 24 h after tMCAO motor performance, assessed by the Bederson score, was slightly impaired, however after 28 days, both treatment groups recovered equally (C). Several behavioural tests were performed over the 28 days observation period, including Grip Strength (D), Rotarod (E) and Adhesive Removal (F). Until the end of the experiment, none of these tests detected any significant differences between platelet depleted and control animals. Grey area: duration of treatment. In case of normal distribution unpaired, two-tailed Student's t-test corrected by Holm-Sidak method was used for statistical analysis, otherwise Mann-Whitney-U test was applied. N = 26/27 (Survival), N = 15/16 (Body Weight), N = 8/8 (Bederson score), N = 17/18 (Grip Strength, Rotarod, Adhesive Removal).