TABLE 1.
L. lactis strains and plasmids used in this study
| Strain or plasmid | Relevant properties | Reference |
|---|---|---|
| Strains | ||
| NZ9800 | 26 | |
| NZ9880 | NZ9800 derivative; ΔptsH | This work |
| NZ9881 | NZ9800 derivative: ΔptsI | This work |
| Plasmids | ||
| pUC19E | pUC19 derivative containing an Ermr gene | 28 |
| pNZ8030 | Lactococcal cloning and expression vector | 7 |
| pNZ9279 | pGEM-T containing a 0.8-kb PCR product derived from the L. lactis ptsHI operon | This work |
| pNZ9280 | pUC19 containing a 4.5-kb SstI-ClaI fragment carrying the ptsH gene and part of the ptsI gene | This work |
| pNZ9281 | pUC19 containing a 3.5-kb EcoRV-BamHI fragment carrying the ptsH and ptsI genes | This work |
| pNZ9282 | pNZ8030 containing the L. lactis ptsH gene translationally fused to the nisA promoter | This work |
| pNZ9283 | pNZ8030 containing the B. subtilis ptsH gene translationally fused to the nisA promoter | This work |
| pNZ9284 | pNZ8030 containing the S46A ptsH gene translationally fused to the nisA promoter | This work |
| pNZ9285 | pNZ8030 containing the S46D ptsH gene translationally fused to the nisA promoter | This work |
| pNZ9286 | pNZ8030 containing the ptsH and ptsI genes translationally fused to the nisA promoter | This work |
| pNZ9287 | pNZ9286 derivative containing the ptsI gene transcriptionally fused to the nisA promoter | This work |
| pNZ9288 | pNZ8030 containing S46A ptsH and ptsI translationally fused to the nisA promoter | This work |
| pNZ9289 | pNZ8030 containing S46D ptsH and ptsI translationally fused to the nisA promoter | This work |
| pNZ9290 | ptsH disruption construct | This work |
| pNZ9291 | pUC19E derivative containing an internal ClaI-PstI fragment of ptsI | This work |