TABLE 3.
l-Lactate dehydrogenase and pyruvate kinase activities of strains used in this study
| Strain | Nisin A induction (μg/ml) | Carbon source | Activity (mean ± SE)
|
|
|---|---|---|---|---|
| Pyruvate kinase (μmol of PEP formed/mg/min) | l-Lactate dehydrogenase (μmol of NADH formed/mg/min) | |||
| NZ9800 (wild type) | Glucose | 3.20 ± 0.57 | 14.2 ± 0.20 | |
| Galactose | 1.67 ± 0.48 | 9.05 ± 0.31 | ||
| NZ9800/pNZ9284 (S46A ptsH) | 10−5 | Glucose | 3.19 ± 0.43 | 13.84 ± 0.39 |
| 10−5 | Galactose | 1.84 ± 0.06 | 9.12 ± 0.65 | |
| NZ9800/pNZ9285 (S46D ptsH) | 10−5 | Glucose | 2.95 ± 0.09 | 12.38 ± 0.23 |
| 10−5 | Galactose | 2.85 ± 0.16 | 12.43 ± 0.90 | |
| NZ9870 (ΔccpA) | Glucose | 0.79 ± 0.78 | 6.32 ± 0.55 | |
| NZ9870/pNZ9285 (S46D ptsH) | 10−3 | Glucose | 0.75 ± 0.26 | 6.42 ± 0.54 |
| NZ9880 (ΔptsH) | Glucose | 2.14 ± 0.53 | 9.87 ± 0.65 | |
| Galactose | 1.45 ± 0.56 | 8.31 ± 0.32 | ||