Skip to main content
. 2022 Jul 27;63(8):25. doi: 10.1167/iovs.63.8.25

Figure 5.

Figure 5.

LGALS3BP upregulated angiogenesis-related factors through the PI3K/AKT pathway. (A) Representative western blotting images and quantification of phospho-PI3K, PI3K, phospho-AKT, AKT, and phosphorylated extracellular-signal regulated kinase (phospho-ERK) in LGALS3BP-silent HMC3 cells (mean ± SD; n = 3/group; nsP > 0.05, *P < 0.05, **P < 0.01, one-way ANOVA). (B) The protein levels of phospho-PI3K, PI3K, phospho-AKT, AKT, HIF-1α, VEGF-A, and MMP-2 in LY294002-treated HMC3 cells (mean ± SD; n = 3/group; nsP > 0.05, *P < 0.05, **P < 0.01; ***P < 0.001, one-way ANOVA). (C, D) Representative images and quantification of in vitro tube formation assays in Matrigel after co-culture with LY294002-treated HMC3 cells (mean ± SD; n = 3/group; ***P < 0.001, one-way ANOVA). Scale bars: 200 µm. (E) Immunoprecipitation of LGALS3BP in HMC3 cells after 24 hours of hypoxia.