Figure 4.

HS-1793 increases protein stability but not p53 transcription. (A) A549 and (B) H460 cells were pre-treated with HS-1793 for 6 h; incubated with CHX (20 µg/ml) for 0, 1, 3, 6, and 9 h; harvested; and analyzed using western blot. Band intensity was quantified by normalizing p53 with GAPDH as a control using the ImageJ program. (C and D) A549 and H460 cells were treated with HS-1793 (C) in a dose-dependent manner for 24 h and (D) in a time-dependent manner (5 µM). mRNA expression of the cells was measured using a quantitative polymerase chain reaction. B2M was used as a control and normalized to obtain a relative quantification graph. Results are expressed as fold change. Data indicate the mean ± standard deviation of independent experiments performed twice. CHX, cycloheximide; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; B2M, beta-2-microglobulin.