TABLE 1.
Bacterial strains and plasmids used
| Strain or Plasmid | Relevant characteristics | Reference |
|---|---|---|
| E. coli strains | ||
| W3110 | F− IN(rrnD-rrnE) | 31 |
| HS200 | W3110 (F′ Tn10 proAB lacIq ZΔM15) | This work |
| XL1-blue | lac (F′ Tn10 proAB lacIq ZΔM15) | 5 |
| EP243 | W3110Δ(lacIOPZYA) exa-1::Mu dI1734(Km lac)a | 31 |
| EP247 | W3110 cadC1::Tn10 | 31 |
| EP314 | EP243 cadC1::Tn10 | 31 |
| AW737 | Wild type for porins, hisG4 thr-1(Am) tonA31 tsx-78 | 19 |
| HS111 | AW737 (ompF ΔompC zei::Tn10) | This work |
| Plasmids | ||
| pBR322 | Cloning vector, Tetr Apr | 3 |
| pCD470 | pGB2-based plasmid expressing a mutant cadC, Spr Smr | 10 |
| pCADA | pBGS18-based plasmid expressing cadA under the control of lacUV5 promoter, Kmr | 28 |
| pCADB | pBR322-derived plasmid expressing cadB under the control of the tac promoter, Tetr Apr | 28 |
| R471a | R factor, Apr | 15 |
a
The exa-1::Mu dI1734 allele is an insertion of Mu dI1734 in cadA, resulting in a CadA− phenotype.