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iviXVII (pdu) and entF expression as a function of iron concentration in the medium. Cultures were grown overnight in iron-free MOPS minimal medium at pH 7.0. The cultures were diluted to OD600 of 0.1 in the same medium grown to an OD600 of 0.4 (mid-log phase). FeSO4 was then added at the given concentration, and β-galactosidase activities were determined after 5 h. Values represent the averages of three independent cultures with a standard deviation of <10% of the mean.
