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. 2022 Aug 2;22:385. doi: 10.1186/s12870-022-03753-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 8 — MdWRKY40 binds to the MdGLU promoter. (A) MdWRKY40 was divided into two fragments, N (N) terminus, and C (C) terminus. (B) The optimal concentration of 3-AT was determined by cloning proMdGLU into the pHIS2 vector. (C) MdWRKY40 interacted with MdGLU promoter fragments as per the Y1H assay. (D) An EMSA analysis revealed that MdWRKY40 binds to the W-box II of the MdGLU promoter. (E) Luciferase reporter (LUC) assays showed the MdWRKY40-mediated activation of proMdGLU. EMSA, electrophoretic mobility shift assay. *P < 0.05. **P < 0.01