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SDS-PAGE analysis of protein fractions obtained during purification of Ncr-His6. Lanes: 1, homogenate from E. coli M15 (pREP4/pQE-Ncr); 2, E. coli M15 (pREP4/pQE-Ncr) after induction with 0.1 mM IPTG; 3, crude extract after cell lysis; 4, soluble proteins passed through the Ni-NTA column; 5, Ncr-His6 after elution from the Ni-NTA column with 250 mM imidazole; M, molecular size marker.
