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HPLC analysis for the determination of the prosthetic group of Ncr. (A) One microliter of supernatant resulting from TCA precipitation of Ncr-His6. (B) One microliter of the flavin standards FAD, FMN, and RF, each at a concentration of 5 μM. The standards gave rise to three clearly distinguishable peaks at 4.5 (FAD), 6.1 (FMN), and 11.1 (RF) min.
