FIG. 8.

PCR and Southern blot analyses of the distribution of the ncr gene among various P. syringae pv. glycinea strains. (A) PCR carried out with primers Ncr-Sph and Ncr-Bgl on total genomic DNA of strains representing six different races of P. syringae pv. glycinea. The arrow indicates the specific amplification product of 1.1 kb. (B) Southern blot analysis of genomic DNA cleaved with EcoRI and probed with ncr. Lanes: M, molecular size marker; 1, P. syringae pv. glycinea, race 0; 2, race 2; 3 and 4, two different strains of race 4; 5, race 5; 6, race 9; 7, race X.