TABLE 2.

Plasmids used in this study

Plasmid	Relevant characteristics	Reference or source
Cosmid 5/III	Tcr, genomic cosmid library clone of PG4180, approximately 35-kb insert in pRK7813	15
pBluescript II SK	Apr, high-copy-number cloning vector	Stratagene
pECos5	Apr, 4.2-kb EcoRI fragment of cosmid 5/III in pBluescript II SK	This study
pMAL-c2	Apr, expression vector for malE translational fusions	New England Biolabs
pQE70	Apr, expression vector for C-terminal His tag fusions	Qiagen
pMAL-Ncr	Apr, contains a 1,132-bp BamHI-HindIII fragment with coding sequence of ncr downstream of the factor Xa cleavage site in pMAL-c2	This study
pQE-Ncr	Apr, contains a 1,078-bp SphI-BglII fragment with the ncr gene truncated at its 3′ end in pQE70	This study