FIGURE 4.

LSD1 expression were elevated during H/R induced injury in vitro. The HK‐2 cells were subjected to hypoxia 12 h and reoxygenation 2, 4 and 6 h. (A) The cell viability was examined by CCK‐8 kit in HK‐2 cells after H/R. (B–C) LSD1 expression was detected by Western blot in HK‐2 cells after H/R, and the quantification was also shown. (D–E) Various concentration of LSD1 inhibitor was employed to examine LSD1 expression, and the quantification was also shown. (F–I) The expression of LSD1 and H3K9me2 was examined by Western blot after inhibition of LSD1 with TCP or si‐RNA against LSD1 (n = 8). The results were expressed as mean ± SEM. *p < 0.05, when compared with the control group. #p < 0.05, when compared with the H/R group