FIGURE 7.

LSD1 aggravates ferroptosis and oxidative stress induced by H/R process via activation of TLR4/NOX4 pathway in HK‐2 cells. The HK‐2 cells were subjected to hypoxia 12 h and reoxygenation 6 h. The HK‐2 cells were transfected with si‐NC or LSD1 for 24 h, and then subjected to H/R, with or without infected with adenovirus carrying TLR4. (A–D) The expression of LSD1 and H3K9me2 was examined by Western blot after LSD1 silence with or without ad‐TLR4, and quantification was also shown. (E‐G) The expression of TLR4/NOX4 was examined by Western blot after LSD1 silence with or without ad‐TLR4, and quantification was also shown. (H‐M) The expression of ASCL4, 4‐HNE, GPX4 and FSP1 was examined by Western blot after LSD1 silence with or without ad‐TLR4, and quantification was also shown. (N‐Q) The SOD, MDA, GSH and Fe2+ levels were detected after LSD1 silence with or without ad‐TLR4, and quantification was also shown (n = 8). The results were expressed as mean ± SEM. *p < 0.05, when compared with the control group. #P < 0.05, when compared with the H/R + si‐NC group. △p < 0.05, when compared with the H/R + si‐LSD1 group