FIGURE 8.

LSD1 activated TLR4 transcriptional activity of TLR4 through removal of H3K9me2 from TLR4 promoter. (A) Promoter sequence of TLR4 (from −2000 bp to +600 bp) was obtained from NCBI public database. (B) HK2 cells were transfected with indicated plasmids carrying Flag‐LSD1 and truncation of TLR4 promoter, and transcriptional activity of TLR4 promoter was detected by luciferase reporter assay. *p < 0.05, when compared with the Flag group. (C) The binding of H3K9me2 to TLR4 promoter was tested by ChIP assay. *p < 0.05, when compared with the con group. (D) mRNA level of TLR4 was assessed by qPCR. *p < 0.05, when compared with the con group. (E) ChIP assay was employed to evaluated the enrichment of H3K9me2 in TLR4 promoter. *p < 0.05, when compared with the con group. **p < 0.05, when compared with the H/R + DMSO group. (F) mRNA level of TLR4 was assessed by qPCR. *p < 0.05, when compared with the con group. **p < 0.05, when compared with the H/R + DMSO group. (G) The binding of H3K9me2 to TLR4 promoter was tested by ChIP assay. *p < 0.05, when compared with the con group. **p < 0.05, when compared with the H/R + NC group. (H) mRNA level of TLR4 was assessed by qPCR. *p < 0.05, when compared with the con group. **p < 0.05, when compared with the H/R + NC group