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. Author manuscript; available in PMC: 2023 Sep 6.
Published in final edited form as: ACS Nano. 2022 Jul 25;16(8):12305–12317. doi: 10.1021/acsnano.2c03219

Figure 1. Characteristics of PSGMR and its derivatives.

Figure 1.

(a) high-performance liquid chromatography (HPLC) and (b-d) Electrospray ionization mass spectrometry (ESI-MS) results of the designed peptide incubation with Mpro. (e) Molecular composition, (f) HPLC, (g) absorption, and (h) fluorescence spectra of PSGMR, PSMR and PMR showed the good purity and solubility enhancement with the decreased fluorescence intensity. (i) Fluorescence spectra and (j) kinetics of PSGMR with different concentration of Mpro and 10 μM GC376 at 590 nm showed the fluorescence increase because of Mpro. (k) Probe specificity of PSGMR with 200 nM different proteins including Mpro, papain-like protease (PLpro), thrombin (TB), bovine serum albumin (BSA), and hemoglobin (HGB). (l) Hydrodynamic sizes, (m) zeta potential values, (n) photographs and transmission electron microscope (TEM) images of PSGMR with Mpro. In panel j, vials contained 10, 50, 100, 200, and 400 μM PSGMR solutions under UV light (365 nm, 16 W). 10 μM of PSGMR, PSMR, PMR, and PyTPE were dissolved in Tris-HCl buffer with 1% DMSO with λex = 405 nm.