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. Author manuscript; available in PMC: 2023 Sep 6.
Published in final edited form as: ACS Nano. 2022 Jul 25;16(8):12305–12317. doi: 10.1021/acsnano.2c03219

Figure 2. Mitochondrial imaging of HeLa cells with PyTPE and PSGMR.

Figure 2.

(a) Confocal laser scanning microscopy (CLSM) images and the normalized fluorescence intensities based on the red dotted lines of HeLa cells with Hoechst 33258, PyTPE and a commercial dye Mito tracker green (MTG) for co-localization imaging. (b) The experimental scheme of HeLa cells after incubation with LPS for 3 h, and PyTPE, PSGMR, Hoechst 33258, and PI. The enlarged portion shows the chemical construction of LPS. CLSM images and average fluorescence intensities of HeLa cells with (c) PyTPE, Hoechst 33258, and PI, (d) LPS, PyTPE, Hoechst 33258, and PI, (e) LPS, PSGMR, Hoechst 33258, and PI. The MTG is activated for mitochondria in the green channel. The PI is activated in the red channel when cells are dead.