FIG. 6.

Epitope mapping of MAb 44-2 and its inhibitory effect on UMP kinase. (A) ELISA was performed with UMP kinase (○) or its fragments (▾, 25-241; ▿, 50-241; •, 113-241; ⊞, 1-180; ■, 1-120; □, BSA) as described in Materials and Methods. The inset shows Western blot analysis with 100 ng of protein, performed as described in Materials and Methods. 1-241 corresponds to the complete UMP kinase. (B) UMP kinase inhibition by MAb 44-2 was performed as described for Fig. 4A. The inset shows Western blot analysis with 100 ng of wild-type (WT) or mutated variants of UMP kinase.