Table 2.

Average Cell Positivity (counts/μm²) on Immunohistochemistry Stainings Summarized as Mean ± SEM.

Control	Bicarbonate
	C	BC	cT	cTB	OT	OTB	LT	LTB
Tumor edge
CD3	0.25 ± 0.06	0.16 ± 0.1	0.16 ± 0.01	0.25 ± 0.1	0.42 ± 0.05	0.43 ± 0.01	0.2 ± 0.1	0.25 ± 0.01
HLA-DR	0.57 ± 0.25	0.35 ± 0.16	0.27 ± 0.09	0.39 ± 0.12	0.55 ± 0.14	0.66 ± 0.09	0.48 ± 0.1	0.52 ± 0.17
CTLA-4	1.72 ± 0.17	0.49 ± 0.1	0.27 ± 0.18	0.19 ± 0.16	0.22 ± 0.12	0.1 ± 0.03	0.68 ± 0.15	0.64 ± 0.15
PD-1	0.97 ± 0.1	0.48 ± 0.1	0.43 ± 0.08	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.21 ± 0.1	0.32 ± 0.04
PD-L1	0.84 ± 0.06	0.45 ± 0.04	0.21 ± 0.06	0.5 ± 0.0	0.34 ± 0.1	0.35 ± 0.12	0.43 ± 0.16	0.42 ± 0.03
Tumor core
CD3	0.37 ± 0.09	0.26 ± 0.08	0.03 ± 0.02	0.26 ± 0.1	0.32 ± 0.07	0.43 ± 0.01	0.33 ± 0.2	0.49 ± 0.3
HLA-DR	0.63 ± 0.05	0.53 ± 0.07	0.29 ± 0.14	0.54 ± 0.1	0.48 ± 0.08	0.7 ± 0.18	0.78 ± 0.1	1.02 ± 0.27
CTLA-4	1.78 ± 0.16	0.7 ± 0.3	0.02 ± 0.0	0.15 ± 0.09	0.02 ± 0.0	0.03 ± 0.02	0.6 ± 0.31	0.58 ± 0.01
PD-1	1.3 ± 0.23	0.7 ± 0.28	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.0 ± 0.0	0.5 ± 0.1	0.0 ± 0.0
PD-L1	1.09 ± 0.2	1.02 ± 0.21	0.3 ± 0.11	1.0 ± 0.0	0.29 ± 0.09	0.54 ± 0.25	0.86 ± 0.24	0.7 ± 0.1

BC = bicarbonate; C = control; cT = conventional transcatheter arterial chemoembolization; CD = cluster of differentiation; cTB = conventional transcatheter arterial chemoembolization + bicarbonate; CTLA-4 = cytotoxic T-lymphocyte–associated protein-4; HLA-DR = human leukocyte antigen DR type; LT = LUMI-bead transcatheter arterial chemoembolization; LTB = LUMI-bead transcatheter arterial chemoembolization + bicarbonate; OT = Oncozene-microsphere transcatheter arterial; OTB = Oncozene-microsphere transcatheter arterial chemoembolization + bicarbonate; PD-1 = programmed cell death protein-1; PD-L1 = programmed cell death protein-1 ligand.