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Expression of IcsA constructs in S. flexneri SC560 (A and B) and E. coli MBG263 (C). Western blot analysis of whole-cell extracts (A and C) and supernatant extracts (B) was performed with monoclonal antibody to IcsA. For each panel, approximately equivalent amounts of protein preparations were loaded in each lane. Apparent molecular masses are indicated, in kilodaltons.
