Table 1. The levels of the variables in this study.

Factors	Levels
	-α	-1	0	+1	+α
A (time, min)	6.59	10	15	20	23.41
B (temperature,°C)	33.18	40	50	60	66.82
C (volume, μL)	242.96	300	550	1000	1067.04