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. 2022 Jul 21;18(7):e1010607. doi: 10.1371/journal.ppat.1010607

Fig 4. Cross-regulation of Zn and Cu stress responses by copY and covR.

Fig 4

Transcripts of sczA (A) and copY (B) were quantified by qRTPCR from cultures of WT, ΔcopY, ΔcovR and ΔsczA mutants supplemented with Zn (0.25 mM) or Cu (0.5 mM) and compared to non-exposed (THB only) controls (n = 4). Absolute transcript amounts were normalized using housekeeping dnaN and generated from standard curves using GBS genomic DNA. Transcripts of sczA and copY were not detected (n.d.) in the ΔsczA and ΔcopY mutant strains, respectively. Quantities were compared using ordinary one-way ANOVA and Holm Sidak multiple comparisons (***P < 0.001).