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. 2022 Jul 15;13:896958. doi: 10.3389/fimmu.2022.896958

Figure 2.

Figure 2

mRNA vaccine manufacturing process. (1) Sequencing and analysis of essential proteins of the virus; (2) The introduction of the plasmid into Escherichia coli and cultured and proliferated; (3) Plasmid extraction, purification, and Enzymatic digestion; (4) in vitro transcription of mRNA; (5) Microfluidic mixing; (6) The process of encapsulating mRNA into lipid nanoparticles (LNPs).