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. 2022 Jul 19;12:726556. doi: 10.3389/fonc.2022.726556

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Copyright © 2022 Liu, Liu, Zhao, Cheng, Liu, Wang, Wang, Lian, Zhu and Gao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects on proliferation and migration of glioma cells by down-regulating GNG12 expression levels. (A) Detection of knockdown efficiency of different siRNA sequences in the glioma cell line LN229. (B) CCK-8 assay to compare the effect on cell proliferation after down-regulation of GNG12. (C) Immunofluorescence assay comparing the positive rate of Ki-67 after transfection with siRNA-NC and siRNA-KD (Magnification: *400). (D) Immunofluorescence assay to analyze the results. (E) Cell scratch healing assay using LN229 cell line to compare the migration distance between GNG12-NC group and GNG12-KD group (Magnification: *200). (F) Results of statistical analysis of cell scratching experiments. (****P<0.0001, ***P<0.001, **P<0.01, *P<0.05).