Figure2.

RP3-326I13.1 was essential for proliferation, migration, invasion and DDP-resistance of LAD cell lines. (a) The expression level of RP3-326I13.1 of the OE-RP3-326I13.1 (RP3-326I13.1 overexpression) A549 cell was higher than that of A549 OE NC cells. (b) The expression level of RP3-326I13.1 of sh-RP3-326I13.1 (RP3-326I13.1 knockdown) A549/DDP cell decreased than sh-RP3-326I13.1 NC A549/DDP cell. (c) We found that RP3-326I13.1 overexpression increased the IC50 values of DDP in A549 cells, while RP3-326I13.1 knockdown significantly decreased the IC50 values of DDP in A549/DDP cells. (d) CCK-8 assay showed that the OE-RP3-326I13.1 group promoted LAD cell proliferation, which was inhibited by treatment with DDP (1 μg/ml), but RP3-326I13.1 overexpression reversed the inhibitory effect of DDP in A549 cells. (e) Sh-RP3-326I13.1 group inhibited the proliferation of LAD cell lines. After treatment with DDP (2 μg/ml), the proliferation of cells was inhibited.(f) We found that the OE-RP3-326I13.1 group promoted clonogenic ability, which was inhibited by DDP treatment, while the opposite was true for the RP3-326I13.1 knockdown group. (g) Cell cycle testing found that after RP3-326I13.1 overexpression, the proportion of cells in G2 + S phase increased, but the proportion of cells in G1 phase also increased. After treatment with DDP, there is no significant difference between G1 and G2 + S phases. (h-j) OE-RP3-326I13.1 group promoted the migration and invasion of LAD cell lines(200 times under the mirror). After treatment with DDP, migration and invasion were significantly lower than those without DDP treatment, but RP3-326I13.1 overexpression reversed the inhibitory effect of DDP, and RP3-326I13.1 knockdown cells produced the opposite result. Cytological experiments were repeated three times .* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ^## P < 0.01, ^### P < 0.001, ^#### P < 0.0001.