Figure3.

RP3-326I13.1 promoted LAD cell proliferation, invasion and DDP-resistance by combining with HSP90B. (a) It showed the electrophoresis diagram of the PCR products. (b) RNA pulldown and silver staining figure of RBP bound to RP3-326I13.1. (c) We carried out mass spectrometry identification and obtained Venn diagrams of different proteins. (d) Interaction potential between HSP90B and the 3’-UTR of RP3-326I13.1 was predicted by RNA-Protein Interaction Prediction. (e-g) RT-qPCR was used to detect HSP90B expression in A549 cells and A549/DDP cells, adjacent tissues and LAD tissues, relief tissues and progression tissues. (h) HSP90B expression was knocked down in A549/DDP cells. (i) Compared with the si-NC group, the si-HSP90B group can inhibit cell proliferation. After treatment with DDP, the cell proliferation is inhibited, and the si-HSP90B+DDP group has a stronger inhibitory effect on A549/DDP cells. (j) knockdown of HSP90B inhibited cell invasion, which was inhibited by treatment with DDP, and knockdown of HSP90B further inhibited invasion. (k) HSP90B knockout promoted apoptosis of A549/DDP cells, while DDP treatment produced more apoptotic cells. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ^### P < 0.001, ^#### P < 0.0001.