FIG. 1.
Genetic organization of the luxO-luxU region of the V. harveyi chromosome. The region encompassing luxO and luxU in the V. harveyi chromosome is shown. Sequence analysis suggests that a partial ORF encoding a V. harveyi uvrB homologue is located upstream of luxO and extends 692 bp into the sequenced fragment. The luxO gene is encoded by bp 1084 to 2445, and the luxU gene extends from bp 2442 to bp 2787. Two other ORFs, designated ORFA and ORFB, reside downstream of luxU and extend from bp 3302 to 3655 and from bp 3682 to bp 4312, respectively. Arrows designate the direction of transcription of each ORF. The extent of the chromosomal deletion in strain JAF78 is also depicted, showing that it encompasses both luxO and luxU. The PstI and MfeI sites used to insert Knr and Cmr cassettes are also shown (see Materials and Methods).