TABLE 1.
V. harveyi strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or feature | Reference or source |
|---|---|---|
| BB120 | Wild type | 6 |
| JAF78 | ΔluxOU-Cmr | 18 |
| JAF483 | luxO D47A linked to Knr | 18 |
| JAF548 | luxO D47E linked to Knr | 18 |
| JAF549 | luxN L166R linked to Knr | 18a |
| JAF536 | luxU::Knr | This study |
| JAF553 | luxU H58A linked to Knr | This study |
| JAF558 | luxN L166R luxU::Cmr | This study |
| JAF552 | luxO D47E luxU::Knr | This study |
| pLAFR2 | Broad host range; mob Tetr | 19 |
| pPH1JI | Broad host range; tra mob | 11 |
| pRK2013 | Broad host range; tra | 15 |
| pALTER | For site-directed mutagenesis | Promega |
| pUC4K | Knr cassette | Pharmacia |
| pPHP45Ω | Cmr cassette | 17 |
| pJAF182 | luxOU in pALTER | 18 |
| pJAF806 | pLAFR2 with luxU::Knr | This study |
| pJAF829 | pLAFR2 with luxO D47E, luxU::Knr | This study |
| pJAF830 | pLAFR2 with luxU H58A linked to Knr | This study |
| pJAF962 | pLAFR2 with luxU H103A linked to Knr | This study |