Table 5.
Results of plasma cytokine assay in healthy controls and NSCLC patients (pg/ml).
| Health controls (n = 8) | NSCLC patients | |||
|---|---|---|---|---|
| Pre-RT (n = 16) | RT (n = 16) | Post-RT (n = 16) | ||
| BLC | 27.41 (11.61–69.61) | 73.63 (12.87–286.32) | 156.24 (16.72–666.31) | 186.29 (20.43–514.35)∗ |
| Eotaxin | 147.89 (109.28–228.22) | 94.84 (56.68–137.3)∗∗∗ | 118.44 (54.95–177.57) | 126.52 (67.84–177.98) |
| Eotaxin-2 | 127.28 (70.63–187.24) | 110.01 (11.28–262.55) | 133.25 (42.01–265.49) | 130.79 (53.21–264.85) |
| G-CSF | 46.73 (3.95–184.06) | 64.04 (0–905.69)∗ | 64.63 (0.25–584.34)∗ | 21.62 (4.04–139.05) |
| GM-CSF | 200.87 (114.71–285.43) | 136.91 (64.75–236.43)∗ | 125.27 (50.87–272.29)∗∗ | 107.9 (39.27–273.09)∗∗ |
| I-309 | 0.72 (0–4.21) | 25.24 (0–331.4)∗∗ | 13.03 (0–130.58)∗∗ | 10.02 (0–51.88)∗ |
| ICAM-1 (×103) | 20.98 (18.54–24.28) | 17.45 (7.5–23.10)∗ | 17.61 (11.31–21.59)∗ | 18.26 (11.50–23.91) |
| IFN-γ | 300.64 (185.68–424.81) | 247.44 (117.81–420.07) | 217.9 (89.21–491.77) | 197.61 (109.57–469.69)∗ |
| IL-1α | 33.94 (20.36–46.56) | 11.3 (1.91–31.71)∗∗∗∗ | 8.03 (0.78–24.01)∗∗∗∗ | 6.62 (0–23.6)∗∗∗∗ |
| IL-1β | 1.11 (0–8.91) | 9.59 (0–150.85) | 4.34 (0–67.69) | 0.98 (0–14.91) |
| IL-1ra | 5.48 (4.04–7.53) | 14.63 (2.54–55.81) | 17.93 (1.67–135.67) | 14.85 (1.23–54.23) |
| IL-2 | 19.15 (10.29–32.66) | 12.32 (5.17–22.37)∗ | 10.43 (4.11–21.41)∗∗ | 9.25 (3.66–18.12)∗∗∗ |
| IL-4 | 81.06 (45.89–127.04) | 32.42 (2.22–117.83)∗∗ | 22.87 (0–64.6)∗∗∗∗ | 19.49 (0.05–59.9)∗∗∗∗ |
| IL-5 | 61.58 (41.33–89.77) | 38.27 (17.96–66.01)∗∗ | 34.44 (14.01–84.83)∗∗ | 31.45 (9.66–65.89)∗∗∗ |
| IL-6 | 83.3 (56.92–115.93) | 81.52 (27.31–153.12) | 74.87 (30.47–222.07) | 66.91 (33.25–95.56) |
| IL-6R (×103) | 2.46 (2.02–3.00) | 2.5 (1.71–3.16) | 2.43 (1.86–3.10) | 2.44 (2.10–2.78) |
| IL-7 | 114.55 (77.97–169.47) | 72.2 (40.25–117.13)∗∗∗ | 67.55 (33.92–116.7)∗∗ | 62.42 (27.52–97.6)∗∗∗∗ |
| IL-8 | 46.16 (29.58–63.42) | 39.08 (13.19–83.77) | 33.66 (9.6–69.15) | 29.44 (3.82–61.95)∗ |
| IL-10 | 28.34 (18.32–39.12) | 21.05 (7.45–37.27) | 20.29 (9.56–42.14) | 17.56 (8.85–39.11)∗∗ |
| IL-11 (×103) | 0.52 (0.32–0.63) | 0.53 (0.21–1.87) | 0.48 (0.17–1.75) | 0.50 (0.22–1.67) |
| IL-12p40 | 46.68 (12.53–86.01) | 52.94 (7.45–292.73) | 28.93 (7.56–137.21) | 50.36 (3.38–236.59) |
| IL-12p70 | 3.63 (2.41–5.29) | 2.99 (0.73–5.3) | 2.95 (1.63–5.27) | 2.16 (0.68–4.8)∗∗ |
| IL-13 | 80.72 (54.01–110.56) | 51.15 (18.9–98.46)∗∗ | 48.23 (19.27–90.37)∗∗ | 41.76 (13.49–89.51)∗∗∗ |
| IL-15 | 157.97 (94.63–205.65) | 128.07 (67.21–234.93) | 107.08 (0–200.38) | 95.17 (30.29–202.59) |
| IL-16 | 28.6 (15.19–38.37) | 58.11 (7.4–380.9) | 28.74 (5.59–76.82) | 20.62 (9.09–45.19) |
| IL-17 | 10.54 (6.36–18.56) | 8.01 (0.91–38.4) | 5.52 (0.28–35.99) | 4.5 (0.06–27.53)∗ |
| MCP-1 | 195.82 (171.53–230.02) | 153.29 (75.72–270.93)∗ | 174.08 (53.52–378.28) | 203.18 (89.98–342) |
| MCSF | 0 (0–0) | 1.05 (0–13.67) | 0.53 (0–3.32) | 0.2 (0–0.99) |
| MIG | 1.03 (0–5.64) | 44.07 (0–499.69) | 43.09 (0–334.09) | 109.6 (0–669.56) |
| MIP-1α | 59.88 (25.68–182.29) | 76.9 (14.56–296.98) | 85.26 (27.62–392.83) | 80.63 (30.64–181.63) |
| MIP-1β | 36.54 (16.33–50.45) | 26.32 (13.74–41.69)∗ | 39.11 (16.59–79.61) | 40.58 (17.45–69.73) |
| MIP-1d (×103) | 4.10 (2.53–5.26) | 3.68 (2.36–5.29) | 3.46 (2.00–5.72) | 3.75 (0.72–5.92) |
| PDGF-BB | 479.88 (392.19–539.18) | 483.86 (343.26–619.91) | 447.45 (326.7–573.83) | 433.7 (215.86–585.86) |
| RANTES (×103) | 1.98 (1.72–2.42) | 2.06 (1.62–2.60) | 2.12 (1.70–2.63) | 2.17 (1.91–2.60)∗ |
| TIMP-1 (×103) | 5.02 (4.54–5.57) | 5.03 (3.96–6.06) | 5.09 (3.97–5.82) | 5.10 (3.82–5.73) |
| TIMP-2 (×103) | 7.11 (5.38–8.50) | 7.11 (5.94–9.23) | 6.90 (4.38–8.36) | 7.35 (6.37–8.76) |
| TNF-α | 794.8 (326.76–1100.16) | 415.58 (132.32–734.35)∗∗∗ | 394.79 (121.71–963.41)∗∗ | 326.07 (97.02–698.34)∗∗∗ |
| TNF-β (×103) | 0.66 (0.30–0.95) | 0.43 (0.065–1.59) | 0.32 (0.068–0.92)∗∗ | 0.32 (0–1.03)∗∗ |
| TNF RI (×103) | 1.78 (0.66–2.49) | 2.68 (1.37–5.28)∗ | 2.75 (1.48–3.84)∗∗ | 2.82 (1.79–3.89)∗∗∗ |
| TNF RII (×103) | 4.25 (3.45–5.45) | 5.64 (3.71–8.24)∗∗ | 5.83 (4.41–8.09)∗∗∗ | 6.20 (4.39–8.32)∗∗∗ |
Data are presented as mean (interquartile range). CCL11: CeC motif chemokine 11; G-CSF: granulocyte colony-stimulating factor; GM-CSF: granulocyte–macrophage colony-stimulating factor; ICAM-1: intercellular adhesion molecule-1; IFN-γ: interferon-gamma; IL: interleukin; MCP-1: monocyte chemoattractant protein-1; M-CSF: macrophage colony-stimulating factor; CXCL9: CXC ligand 9; MIP: macrophage-inflammatory protein; PDGF-BB: platelet-derived growth factor BB; RANTES: regulated on activation normal T expressed and secreted chemokines; TIMP: tissue inhibitor of metalloproteinases; TNF: tumor necrosis factor; TNFR: tumor necrosis factor receptor. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 compared with healthy controls.