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. 2022 Jul 26;2022:4061713. doi: 10.1155/2022/4061713

Figure 6.

Figure 6

AHCY overexpression enhanced- while its knockdown weakened the inflammation and oxidative stress in vitro. (a, b) Western blot was used to examine the effect of AHCY shRNA on the expression of COX-2 and iNOS in SW620 cells, respectively. (c, d) Western blot was used to examine the effect of AHCY overexpression on the expression of COX-2 and iNOS in DLD-1 cells, respectively. (e, f) ELISA was used to examine the effects of AHCY shRNA on the levels of IL-1β and IL-6 in the supernatant of SW620 cells, respectively. (G-H) ELISA was used to examine the effects of AHCY overexpression on the levels of IL-1β and IL-6 in the supernatant of DLD-1 cells, respectively. (i, j) Effect of AHCY knockdown on the levels of MDA and GSH in SW620 cells, respectively. (k, l) Effect of AHCY overexpression on the levels of MDA and GSH in DLD-1 cells, respectively. (m, n) Effect of AHCY knockdown on the activities of GSH-Px and SOD in SW620 cells, respectively. (o, p) Effect of AHCY overexpression on the activities of GSH-Px and SOD in DLD-1 cells, respectively. Data are expressed as the mean ± SD (n = 3). ##p < 0.01 and###p < 0.001 versus the control shRNA group; ∗p < 0.05,  ∗∗p < 0.01, and∗∗∗p < 0.001 versus the control vector group.