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. 2022 Jul 26;2022:4061713. doi: 10.1155/2022/4061713

Figure 8.

Figure 8

AHCY overexpression activated the Hh signaling whereas AHCY knockdown inactivated the Hh signaling in vitro. (a) Western blot was used to examine the effect of AHCY shRNA on the expression of Hhip and Patched in SW620 cells. Representative blots are shown with densitometry (n = 3). (b) Quantitation of the result of western blot. (c–f) Western blot was used to examine the effect of AHCY shRNA on the expression of cytoplasm Gli 1 and nucleus Gli 1 in SW620 cells, respectively. Representative blots are shown with densitometry (n = 3). (g) Western blot was used to examine the effect of AHCY overexpression on the expression of Hhip and Patched in DLD-1 cells. Representative blots are shown with densitometry (n = 3). (h) Quantitation of the result of western blot. (i–l) Western blot was used to examine the effect of AHCY overexpression on the expression of cytoplasm Gli 1 and nucleus Gli 1 in DLD-1 cells, respectively. Representative blots are shown with densitometry (n = 3). Data are expressed as the mean ± SD (n = 3). ##p < 0.01 and###p < 0.001 versus the control shRNA group or control vector group.