TABLE 2.
Primer | Sequence (5′ to 3′)a | Amino acid sequence | Avg GC content (%) |
---|---|---|---|
prSJS247 | ACMGARTTCTWCGGMGARTTCGGMTCKGGMAA | TE F F GEFGSGK | 51.6 |
prSJS252 | ACSGARKTSTWCGGSGARTTCGGSKCSGGSAA | TE V/F Y/F GEFGSGK | 62.5 |
prSJS254 | ACWGARTTYKYWGGWGARTTYGGWWSYGGWAA | TE F F/A GEFGSGK | 43.8 |
prSJS248a | RTCKGGKYTKGCKGAKACYTGRTTKGT | TNQV S A N/R PD | 51.9 |
prSJS253 | GTCSGGGTTSGMSAMSACCTGGTTSGT | TNQV A/S A/S N PD | 63.0 |
prSJS255 | RTCWGGYCTWGCWGCWACYTGRTTWGT | TNQV A A R PD | 48.1 |
Conforms to the standard DNA alphabet as follows: W, A or T; Y, T or C; S, G or C; M, A or C; K, T or G; R, A or G; B, T or C or G; H, A or T or C; D, A or T or G; V, A or C or G; N, A or T or C or G.