Skip to main content
. 1999 Feb;181(3):907–915. doi: 10.1128/jb.181.3.907-915.1999

TABLE 2.

Oligonucleotide primers for PCR

Primer Sequence (5′ to 3′)a Amino acid sequence Avg GC content (%)
prSJS247 ACMGARTTCTWCGGMGARTTCGGMTCKGGMAA TE  F   F  GEFGSGK 51.6
prSJS252 ACSGARKTSTWCGGSGARTTCGGSKCSGGSAA TE V/F Y/F GEFGSGK 62.5
prSJS254 ACWGARTTYKYWGGWGARTTYGGWWSYGGWAA TE  F  F/A GEFGSGK 43.8
prSJS248a RTCKGGKYTKGCKGAKACYTGRTTKGT TNQV  S   A  N/R PD 51.9
prSJS253 GTCSGGGTTSGMSAMSACCTGGTTSGT TNQV A/S A/S  N  PD 63.0
prSJS255 RTCWGGYCTWGCWGCWACYTGRTTWGT TNQV  A   A   R  PD 48.1
a

Conforms to the standard DNA alphabet as follows: W, A or T; Y, T or C; S, G or C; M, A or C; K, T or G; R, A or G; B, T or C or G; H, A or T or C; D, A or T or G; V, A or C or G; N, A or T or C or G.