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. 2022 Aug 2;5:781. doi: 10.1038/s42003-022-03743-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Correlation between Cdkn2a (p16) mRNA levels at 24 h after irradiation with 0, 6, or 9 Gy and the percentage of SA-β-gal positive cells at 5 days after irradiation in male and female Nf1−/− DNp53 astrocytes (n = 15/sex). b Correlation between Cdkn1a (p21) mRNA levels at 24 h after irradiation with 0, 6, or 9 Gy and the percentage of SA-β-gal positive cells at 5 days after irradiation in male and female Nf1−/− DNp53 astrocytes. Male correlation: r = 0.59, p = 0.0212; Female correlation: r = 0.79, p = 0.0005 (n = 15/sex). c Correlation between the Cdkn1a/Cdk2 mRNA ratio at 24 h after irradiation with 0, 6, or 9 Gy and the percentage of SA-β-gal positive cells at 5 days after irradiation in male and female Nf1−/− DNp53 astrocytes. Male correlation: r = 0.53, p = 0.0427; Female correlation: r = 0.80, p = 0.0003 (n = 15/sex). d Representative western blot images of p21 and Cdk2 5 days post-irradiation with 0, 3, 6, or 8 Gy. One male and one female Nf1−/− DNp53 cell line are shown. Molecular weight markers (MW): p21 (15 kDa), Cdk2 (30 kDa). e Correlation between the p21/Cdk2 protein ratio at 24 h after irradiation with 0, 3, 6, or 8 Gy and the percentage of SA-β-gal positive cells at 5 days after irradiation in male and female Nf1−/− DNp53 astrocytes. Male correlation: r = 0.30, p = 0.0997, female correlation: r = 0.52, p = 0.0029, male slope vs female slope: p = 0.0627—indicated by bracket (n = 31/sex). f Correlation between the p21/Cdk2 protein ratio 5 days after irradiation with 0, 3, 6, or 8 Gy and the percentage of SA-β-gal positive cells at 5 days after irradiation in male and female Nf1−/− DNp53 astrocytes. Male correlation: r = 0.40, p = 0.0239, female correlation: r = 0.65, p < 0.0001, male slope vs female slope: p = 0.009—indicated by bracket (n = 31/sex).