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. 2022 Jan 21;29(8):1500–1512. doi: 10.1038/s41418-022-00938-9

Fig. 2. The CASP8(ΔE385) compromises apoptosis, particularly switches TNF-α induced apoptosis to necroptosis.

Fig. 2

A Western blotting analysis of the indicated protein in primary WT and Casp8ΔE385/ΔE385 thymocytes which were treated with FasL (Jo-2) (1 μg/ml) for the indicated time. The data are representative of three independent experiments. B Primary WT and Casp8ΔE385/ΔE385 MDFs were treated with GSK’872 in different concentration for the indicated time respectively. Bars, mean ± SD. P values above the asterisk (unpaired, two-tailed t test) *p < 0.05, **p < 0.01, ****p < 0.0001. C Western blotting analysis of protein expression of caspase-8, cleaved caspase-8, cleaved caspase-3 and GAPDH in primary WT and Casp8ΔE385/ΔE385 MDFs which were treated with GSK’872 (20 μM) for the indicated time. D Mouse survival curve of 8- to 12-week old mice after challenged by anti-Fas antibody (Jo-2, 0.5 μg/g, i.v.). F, female. P values alongside the asterisk, two-sided Log-rank (Mantel-Cox) test, **p < 0.01. E The alanine transaminase (ALT) and aspartate transaminase (AST) levels in serum of the 16-week old WT, Casp8ΔE385/ΔE385 mice 2.5 h after anti-Fas injection. Bars, mean ± SD. P values above the asterisk (unpaired, two-tailed t test), **p < 0.01. F Primary WT and Casp8ΔE385/ΔE385 MDFs were treated with TNF-α (20 ng/ml), TNF-α + Smac (1 μM) (TS), TNF-α + CHX (20 μg/ml) (TC) for 5 h. Bars, mean ± SD. P values above the asterisk (unpaired, two-tailed t test) ***p < 0.001. G Immunoblotting of the indicated protein expression in primary WT and Casp8ΔE385/ΔE385 MDFs which were treated with TNF-α (40 ng/ml) +CHX (40 μg/ml) (TC) for the indicated time.