Fig. 4.

Inhibition of miR-214-3p alleviates ferroptosis in cisplatin -induced TCMK-1 Cells. A Flow cytometry assays were performed to show the cell death. B Statistical analysis was used to show the levels of cell death. The levels of ROS (C), iron (D), and GSH (E) in TCMK-1 cells. F qRT-PCR analysis of GPX4, ACSL4, and SLC7A11 expression in TCMK-1 cells. G, H Western blot analysis of GPX4, ACSL4, and SLC7A11 expression in TCMK-1 cells. Data are expressed as the mean ± SD. n = 5 per group. ^*P < 0.05 and ^∧P > 0.05 vs. con antagomir NC group, ^#P < 0.05 vs. antagomir NC group treated by cisplatin, one-way ANOVA