Fig. 5.

CWP activated the Nrf2/HO-1 signaling pathway in HS rats. A Representative figures of IHC results of Nrf2. Green arrows indicate enhanced Nrf2 staining intensity in the nucleus. B IHC scores of Nrf2. C The expression of total Nrf2 in hepatocytes was assessed using a western blotting assay. β-Actin was used as an internal reference protein (D). E, F The expression of HO-1 was investigated using a western blot assay. * Representative significance is based on the comparison with the HS group, whereas # representative significance is based on the comparison with the control group. Data are presented as the mean of at least three determinations ± SEM. * or # p < 0.05, ** or ## p < 0.01, and *** or ### p < 0.001