Fig. 2.

ACY-1215 inhibits the growth of CML cells. (a) ACY-1215’s chemical structure. (b) CCK-8 experiment was performed to determine the cell viability of CML cells that had been exposed to a range of ACY-1215 concentrations. (c) The proliferation potential of CML cells treated with ACY-1215 was determined using a colony formation assay. Colony numbers and sizes were counted and photographed 7 days later. (d, e) Cell cycle distributions of CML cells were examined using flow cytometry after being treated with ACY-1215 for 48 h. Cell cycle-related proteins were investigated using a western blot assay. **p < 0.01, ***p < 0.001, ****p < 0.0001