Fig. 5.

ACY-1215 induces apoptosis and cell cycle arrest by ROS generation. (a, b) CML cells were treated with ACY-1215 for 48 h. Then ROS production of each group was detected by the DCFH-DA fluorescent probe. Fluorescence intensity was measured by flow cytometry. ROS was scavenged by NAC, a ROS inhibitor. NAC was applied to CML cells for 1 h before cells were treated by ACY-1215 for another 24 h. Apoptosis rates (c, d) and cell cycle distributions (e, f) were detected by FCM. *p < 0.05, **p < 0.01