Fig. 6.

ACY-1215 inhibits CML cells by ROS/PTEN/Akt pathway. (a, b) The expression of PTEN in mRNA and protein level were confirmed using Q-PCR and western blot, respectively. p-Akt expression of each group was examined by western blot. (c) PTEN location was detected by immunofluorescent and photographed by confocal microscope. (d) SC79, an Akt activator, was used. CCK-8 was used to determine the cell viability. (e) PTEN expression of mRNA was determined by Q-PCR. (f) The protein level of PTEN was examined using western blot