Fig. 4.

Canonical Interactions of Hsp70 NEFs are highly conserved. A NEF interaction sites are mapped onto the Hsc70 NBD (PDB 1HX1) and color coded by NEF. Binding sites shared by 2 or more NEFs are colored purple. B Domain architecture of the 5 Bag family NEFs shows significant variance outside of the C-terminal Bag domain. C The co-crystal structure of the human Bag1 Bag domain (green) and Hsc70 (gray; PDB 1HX1) highlight an important network of electrostatics stabilizes the PPI interface. Conservation of these residues across Bag family members is shown below (C-terminal Bag domain was used for Bag 5) from a CLUSTALW multiple sequence alignment