Fig. 5.

Bag family NEFs contain sequences predicted to bind Hsp70’s SBD and displace clients. A Sequences predicted to bind Hsp70 SBDβ within the Bag proteins. Briefly, the ChaperISM python script was used to search human Bag protein sequences in both quantitative mode (cutoff = 2.7) and qualitative mode (cutoff = 0.2). Sequences were only included if they met cutoff thresholds in both quantitative and qualitative modes, and were found outside of the Bag domain. B Model for how a representative BAG protein, Bag3, might use both its canonical Bag domain and its non-canonical, pseudo-substrate motif to interact with two separate sites on Hsp70. In this model, the pseudo-substrate acts as a “release domain” to promote client release from the Hsp70 complex