Fig. 5.

Effects of MitoQ and mdivi-1 on cellular injury, mitochondrial membrane potential, ROS apoptosis rats in H9C2 cells exposed to HG/HF-HR. HG/HF, high glucose and high fat (30 mM glucose, palmitate 300 μM); HG/HF-HR: H9C2 cardiomyocytes were cultured with high glucose and high fat and were subjected to 4 h of hypoxia followed by 2 h of reoxygenation; HG/HF-HR + MitoQ, cells were administrated with MitoQ 2 h before hypoxia; Mdivi-1, cells were administrated with MitoQ 2 h before hypoxia and mdivi-1 24 h before hypoxia. Apoptosis rate (a, b), cell viability (c), mitochondrial membrane potential (d), and ROS (e) were detected by representative kits, scale bar 100 μm. All values are presented as the mean ± SD, n = 5/group. ^∗P < 0.05 versus HG/HF group; ^#P < 0.05 versus HG/HF-HR group; ^&P < 0.05 versus HG/HF-HR + MitoQ group