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. Author manuscript; available in PMC: 2022 Aug 3.
Published in final edited form as: J Bone Miner Res. 2019 Jul 30;34(9):1753–1766. doi: 10.1002/jbmr.3756

Figure 4. Becn1 cKO mice exhibited increased cortical bone thickness but diminished trabecular bone mass.

Figure 4

(a) Photographs of Becn1 WT and cKO mice. (b) Quantification of body weights at 6th month (n = 5 WT, n = 12 cKO, male). (c) Photographs of spleens from Becn1 WT and cKO mice. (d) Quantification of spleen at 6th month (n = 5 WT, n = 12 cKO, male) normalized to the body weights. (e) µCT of the femurs from Becn1 WT and cKO mice. (f) Quantification of femur lengths measured at 6th month (n = 5 WT, n = 12 cKO, male). (g) µCT of the femur cross-sections in Becn1 WT and cKO mice. (h) Quantification of femur cortical bone thickness measured at 6th month (n = 5 WT, n = 12 cKO, male). (i) µCT of the trabecular bone from Becn1 WT and cKO mice. Quantification of BV/TV (j), trabecular thickness (k), trabecular number (l), and trabecular spacing (m) measured at 6th month (n = 5 WT, n = 12 cKO, male). (n) H&E staining of the femur head. Bar = 200 µm. (o) TRAP staining in the femur trabecular bone. Bar = 100 µm. (p) Quantification of TRAP+ osteoclasts per bone perimeter. (q) TRAP staining in the femur cortical bone. Bar = 100 µm. (r) Quantification of the TRAP+ osteoclasts per bone perimeter.