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Gel shift assay comparing the DNA-binding abilities of wild-type H-NS and altered T108I H-NS. Each lane contains 1.6 pM fimB promoter DNA and the indicated amount of either wild-type or T108I H-NS. Reaction mixtures were incubated, electrophoresed on a 1% agarose gel, and stained with ethidium bromide (9). M, ΦX174 marker.
